DETAILS – Pfizer Vaccine Becomes DNA in The Human Liver Cells Huh7. (In-vitro Swedish Study) | DrBeen Medical Lectures | Mon, Feb 28, 2022 (Video with References)
- Mar 4, 2022
- 2 min read
Content Source/Owner:
Drbeen Medical Lectures
~ 1 hr
Summary:
An accepted and published Swedish study demonstrates for the first time the presence of Pfizer-BionTech vaccine DNA in the cell.
It is reverse transcribed by LINE-1 proteins of the cells. They are not sure if it gets integrated in our DNA or not. Let’s review the details of their method.
Chapters
introduction
Link to the summary
Review of the reference material
Cell preparation for the vaccine
Vaccine addition to the cells
RT-PCR for find RNA and then DNA
Imaging of the cells and the LINE-1 protein, ORF-1 protein, and the DNA
Measuring the volume of proteins in the cytosol and the nucleus
DNA purification and verification
Results
LNP enters the liver cells efficiently
LINE-1 gene is expressed
ORF-1 found in cytosol and the nucleus
DNA from the vaccine found in the cell
Questions not answered
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URL list from Friday, Feb. 25, 2022
CIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML
Frontiers | Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines | Genetics
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URL list from Monday, Feb. 28, 2022
CIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML
LINE-1 protein localization and functional dynamics during the cell cycle | eLife
Time evolution of PEG-shedding and serum protein coronation determines the cell uptake kinetics and delivery of lipid nanoparticle formulated mRNA | bioRxiv
Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues – PubMed
No evidence of SARS-CoV-2 reverse transcription and integration as the origin of chimeric transcripts in patient tissues – PMC
Overview of Reverse Transcription – Retroviruses – NCBI Bookshelf
Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus | Nature Microbiology
Chimeric RNA – Wikipedia
Benchtop Automation for Immunohistochemistry (IHC), ISH & Special Stains (SS) – NanoMtrx 300 – YouTube
Schrödinger’s cat – Wikipedia
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Disclaimer:
This video is not intended to provide assessment, diagnosis, treatment, or medical advice; it also does not constitute provision of healthcare services. The content provided in this video is for informational and educational purposes only.
Please consult with a physician or healthcare professional regarding any medical or mental health related diagnosis or treatment. No information in this video should ever be considered as a substitute for advice from a healthcare professional.
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